Today was a shorter day. I previously made a miniprep culture to isolate plasmid from E.Coli. I drew 10mL LB Broth into 4 test tubes and drew up a colony and dipped it into a test tube. Repeated 4 times. Overnight, the prepared culture amplifies the E.Coli and DNA to be harvested. So, today, I resuspended the culture using a centrifuge, four different buffers for each sample, and supernatant. By the end, I moved the sample to a microcentrifuge tube and drained/isolated the DNA to the bottom. Then my time in the lab was cut short before a friend gave me a tour of UNC.
