Day 1

I started by purifying a ligated DNA specimen from my running digest experiment. Then, I used electroporation to transfer the DNA samples into ecoli cells and placed them in a warm room to amplify. Then, my research technician taught me how to use cell imaging/screening to determine if cells are contaminated by a virus. Finally, I was taught how CRISPR edits CF-infected DNA and how to use a sequence editing software to simulate which enzymes could cut DNA sequences in largely different places to make cut DNA strands easy to compare. My next step is to make sure the DNA specimen properly amplified and test my chosen enzyme through a new digest.