Day 4, Opiates, Amphetamines, Cocaine, and more.

Last day at Integrated Pain Solutions! Today was all about analysis of our sample tests from yesterday. Since we did two test runs yesterday and inputted both into the LCMS for readings, we analyzed both.

The first one was four samples. These were real patient samples, but the names were removed. First, we went through the Immunoassay test results from yesterday afternoon’s tests and we marked which positive results were consistent with the drugs that the patient was prescribed and which were inconsistent with what the patient was prescribed. After marking all of these, we looked at the results from the comprehensive confirmation test. This test is much more accurate than the Immunoassay, so it helped us refine the results and eliminate false positives. Based on this, we were able to say with some certainty what drugs the patient was taking and whether or not they were following their prescriptions.

Then, we went over the lab protocol for unpacking new supplies. In this case, we watched the QC liquids be unpacked and then labeled correctly.

Later, we ate lunch. Panera!

Then, we repeated a similar analysis on another run that we had ourselves prepped the day before. We did a process similar to the one described above, but we focused more on the comprehensive confirmation test. This test allowed us to again see if certain patients were taking their prescribed drugs and nothing else. Then afterward we compared the results that we analyzed and the conclusions we drew to the Immunoassay test results.

In the end, we said bye to all of the people who helped us and guided us through the past four days! It was an amazing experience.

PS, So this is something I probably shouldn’t be telling you, but I am. Law enforcement has no significantly appreciable ability to tell the difference between a hemp product and a marijuana product. If the police find marijuana in an individuals car, and it is labeled as hemp, or the individual identifies it as hemp, the police do not have probable cause to arrest that individual. They can, however, drug test that individual and arrest him or her if the drug test comes up positive for THC. However, if the drug test is clean, the police have no ability to tell the difference between hemp and marijuana.

ILS Day 4

From morning to afternoon, today was a day filled with data analysis. We began the workday at 10:00 AM sharp by analyzing the 4 fake patient samples–unceremoniously tagged as 6001, 6003, 6007, and 3184–against both the immunoassay and mass spectrometer results. For most of the patients, the immunoassay results were as expected: they matched the patient prescriptions, giving us a sigh of relief. However, some of the results showed that patients were potentially alcoholic (a presence of ethyl sulfate, or EtS values) or opiate addicts (a presence of morphine despite a lack of a prescription). One of the lab technicians told us that around 30 or 40% of tests don’t come out as per what is expected, so even though our patients were fake, I still believe that this mock test speaks volumes about North Carolina’s ongoing prescription drug abuse crisis.

We were interrupted by a new shipment of quality control samples, which are run on the mass spectrometers to make sure they are in working order. We took advantage of this moment to examine the proper lab procedure for unboxing new lab chemicals, and we shadowed one of the technicians as she meticulously unpacked the refrigerated box, carefully labeled the samples inside, and stowed them away inside the refrigerator.

As this was our last day, time seemed to pass faster than ever, and before we knew it, it was time for a lunch break. Panera Bread was the choice for the day, and a few rounds of foosball suppressed our growling stomachs for the moment.

After a Cuban Sandwich and a comforting bowl of Mac & Cheese, it was time to get back to work. Likewise, we did further mass spectrometer analysis, but this time, it was on a different piece of software. Although we had to leave before we were finished with our analysis, Adam showed us the results, and we were quite accurate! Not bad at all for a second time.

We spent some time giving some heartfelt goodbyes and last words, and we walked out of the doors of Integrated Laboratory Solutions for the final time. This week, I met many bright, creative, and amazing minds, and I would like to thank Dr. Zhong, Adam, Ashleigh, Krystel, Jelani, and Dr. Jay for all the good memories from these past few days.

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