Day 4 @CAAE

The SRP samples analysis that I set up and ran yesterday on the quAAtro came out after we left yesterday. All of the initial setups that affect the rest of the run tested perfect and or right in the range of what they should be.

Today I was shown the process and set up for chlorophyll a filtration.

The process is similar to TSS filtering, in that you concentrate on a known volume of sample onto a filter sheet and use all the same materials. This process measures the amount of algal biomass in a water system. Chlorophyll a is a photosynthetic pigment found in plants and algae and has to be tested for in the dark with only green lights to illuminate without heat or bright lights that are usually on.

This is what the datasheet looks like for five samples taken from Oak Hollow and High Point reservoirs.

Day 1 @CAAE

Welcome to the NC State Center For Applied Aquatic Ecology!

Here are some machines that I learned how to use today as well as some important processes to remember:

Testing template for silica in water and seawater

High purity water machine

quAAtro machine flushing and stabilizing before analyzing [the quAAtro machine is also referred to as the Continuous-Flow Injection Auto-Analyzer] (see attached link below)

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32 silica samples taken from falls lake for a student working on her PhD

Testing for total suspended solids from falls lake with labeled water samples and glass microfiber filters

quAAtro machine analyzing silica samples from falls lake (see attached link below)

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TSS filtering in process (see attached link below)

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Data recorded from the filtering- includes filter paper number, filter paper weight in milligrams, volume of water added, and sample name. The filter and residue weight will be measured Thursday after the filter paper is dried in the oven at 104.1°C. The samples included 3 field duplicates usually meaning they were collected using the same collecting methods and for every ten samples, we did a lab duplicate to see if our filtering methods yielded consistent results. Gathering all the data from filtering took 3 hours, which did not include weighing 30 glass microfiber filters before which took another full hour!

These are the glass microfiber filters after filtering the lake samples

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