DAY 3: Oops.

Day 3 in the Sheikh lab began with a bang, or should I say a splat? In the morning I watched Matt, one of the research technicians, complete a series of protocols to isolate RNA from a human tissue sample. However, on the way from the fume hood to the walk-in fridge, Matt dropped one of the samples and about half of it splattered out across the floor. We had to stop what we were doing and carefully clean up the mess with ethanol to take care of the potential safety hazard. Even though we laughed about the silly mistake, it brought up a good point: accidents happen all the time in the lab, and what’s most important is how you choose to deal with them. Even though Matt lost over half of the treated sample, he decided to continue processing it anyways, because any amount of tissue can provide helpful data. If you decide to throw out the sample after you’ve already done a lot of work with it, you’re just wasting resources. The spill also brought up the necessity of the taking careful notes during your experiment, so that when you get inconsistent or abnormal data, you can come up with an explanation for what might have happened. For example, once the isolation process was completed, the sample that spilled would have significantly less RNA in it, so in order to explain the results, you’d have to look back and remember that you lost half of the sample.

 

 

TRIzol, a chemical buffer used in the homogenization process
The human tissue samples being kept cool in an ice bucket
Centrifuging the tiny samples in the giant walk-in fridge!
A view through a microscope of artificially grown cells called colonoids. I’ve just started learning about what the lab’s doing to grow these cells and do experiments with them, and I’m excited to discover more!

Day 3

I spent the third day of the work experience program learning about marketing and sales and SAS. I arrived at building C at around 9:00, and started off the day by getting a tour around the building. After an hour and a half of walking around and having the opportunity for a Q&A, we met Holton Ramoin, a worker in sales at SAS. We shadowed him for the rest of the day, and he got to teach us the ropes, and really put us through a day in the life of a SAS salesperson. Most of the day we were in his office, and he made phone calls, sent emails, and reached out to different people that may have had an interest in using SAS analytics in their company. He also showed us how they try to expand their presence within a company, and everything that they do to try and expand their brand. Tomorrow, I am excited to see a different aspect of SAS, as I will be in building Q learning about human resources.

Day 2

My second day at the state legislative building was a very busy one. We started off the day by attending a Board of Education meeting so that we could watch the implementation of legislation that Ms. DeVivo had gotten passed a few weeks prior to my arrival. Following the meeting, we went down to the quad and helped Ms. Sams (DeVivo’s partner) set up for a lunch catered by the pit for 500 people. The lunch invited all senators, reps, and optometrist to join so that they could generate discussion about the optometrist bill that we lobbied for the day prior. And following lunch, we met with a client from Riskcor to talk about upcoming legislation involving immigrants. We took the client to a couple of senators and reps (including the president pro-tempore and the majority leader) so that he could express his concerns with the legislation, and try to win their support.

One thing that I learned during my day is the importance of making connections. While shadowing Ms. DeVivo, I noticed that she had very good relationships with pretty much all of the officials. She knew pretty much everyone in the buildings, and was genuine friends with them. By developing these friendships, it became a lot easier to do her job because they knew that they could trust her. And while she was fairly liberal, most of her really good friends were conservative. While I assumed that there would be conflict because of party, there really was not because she said that she has a long history of being fair, and that goes a long way. As she said, people remember everything. If you mess with someone, they will fight back. Making friends is a whole lot more effective than burning bridges.

Day 1

For the first day of my work experience, I spent the day walking around the NC State Legislative building with two lobbyists – Ms. Laura DeVivo and Ms. Angel Sams. They are partners at their firm, DeVivo and Sams, and I got to spend the day walking around with them and watching them meet with reps and senators to pitch their upcoming legislation. The first day, we focused a lot on a specific bill regarding Optometrists. We were fighting on behalf of their clients to allow Optometrists to complete level 1 (very simple) surgeries without needing to go to years of medical school. We started off the day meeting with two senators who were in charge of drafting the bill to help clarify language in it, and then we started meeting senators and reps that were on the fence so that we could gain their support. Throughout the day, I probably met about 15 senators and reps, and it was very interesting to see their different opinions and personalities. Ms. DeVivo said that the building was like a high school: you have many of the cliques, a few popular people, the a couple bullies, and the few that keep to themselves. After doing her job for so long, she really has learned how to get to each, and change her lobbying strategy depending on the person that she was meeting with.

Day 3

Calendar for the session

I had a painful start to my day, as soon as I got out of my car, I hadn’t even opened the door all the way, I was stung by a flying insect I didn’t even get a good look at, and my hand swelled up and turned red immediately. I wandered around the building until I found the cafeteria and got ice (and some weird looks). While this was no means a learning experience it was certainly a highlight of my day. The only thing happening that day was another session, where the House of Reps would be voting on a few bills, and eventually the budget. As I took my seat, the Speaker informed everyone that the session would most likely go on until midnight, as they have to nitpick the entire budget, so I only saw the discussion on the individual bills. The bills on the docket included important issues, like one on the sex offender registry and a telecommunications box that was a potential carcinogen. To my surprise, however, the most contentious debate was over the bill I mentioned in day 1, about interior designers and architects. So many reps would ramble on about the issue, blowing it, in my opinion, out of proportion. I spoke with Representative Martin afterwards about it and he agreed that many important issues don’t get the debate they deserve because so much focus is on industry-related bills, and from what the reporter told me yesterday, this often has to do with which side (interior designers or architects) have the most money or influence in the General Assembly. Outside of the very heated debate about windows and low bearing walls, the members seemed pretty friendly with each other. One pulled out a basket of fruit to share, another said there was donuts in his office anyone could grab. And another made a point of personal preference, stood up, and gave a speech about his fellow rep and to wish him a happy birthday, and told everyone that “he loved chocolate”, after which everyone who had chocolate candy on their desk gave him some of theirs. It was honestly the cutest thing I’ve ever witnessed. Although I was annoyed by the interior designer bill, it was nice to see how nice the 120 members could be to each other.

Day 3

Today was a pretty quiet day at the office. When we showed up in the morning we had a quick meeting with a woman named Morgan, and then she gave us an assignment that took us the majority of the day to finish. We moved rooms and ended up working at the “intern desks,” which meant that we finally got to formally meet one of the two other college interns that were working at French West Vaughn. I never actually caught her name but she was going into her senior year at UNC for marketing and was interning at FWV all summer. We talked to her for a little while about how she got into marketing and how she ended up with her internship. What was interesting was that she said she had no idea that she would end up in marketing when she was our age, and that when she was in high school she wanted to be a marine biologist. She sounded a lot like Rich, the Artistic Director for FWV that we met the day before. Rich also said that he never thought he would be in marketing and design when he was younger. He had told us that he wanted to be a teacher when he started college, but then slowly moved away from teaching and into graphic design as time went on. I guess both Rich and the other Intern we met just show that you don’t necessarily have to know exactly what you want to do going into college because even if you do, your interests could easily change as time goes on.

Intern desks

Day 3: WOW!

I’ve been told my posts are too lengthy, so I’ll try to condense a very busy day and touch on what I feel were the most exciting points. It’s difficult, as were exposed to so many different projects today!
First, a follow up on the cement experiment we set up on the first day: we got great results, and a graph to go with it! The results were aligned with our expectations. Sugars are a retardant in the hydration process of cement and salts are a catalyst. I was shocked to see the difference between the samples with 1% maltodextrin (sugar) and 1% calcium chloride (salt). The maltodextrin sample was still so fluid that it could be easily stirred and poured (see picture). The calcium chloride sample, however, was rock solid – there would have been no way to get it out except cutting open the container (see picture). I did learn that a sample with 0.05% sugar can retard the process by 12 hours, and since we used 1.00%, it could be weeks before our sample sets.
Graph showing the different rates at which cement mixtures hydrated
Hardened cement sample with salt
Fluid cement sample with sugar
The main activity we did today was making WOW particles – water in oil in water particles. They are essentially delivery devices, and the specific WOW particles we made are used for delivering substances into oil wells. The core water, which we colored with food coloring, the second layer is a monomer that serves as the oil, and the third later is water. The “fusing” of the layers, or emulsion, is then set off by shining a UV light on it (see picture). The final step was to put the particles we made under a microscope and look at what we made – cute little spheres! (see pictures)
UV light being shined on mixture
Putting the sample on the microscope
WOW particles under the microscope
Speaking of microscopes, we got to observe an amazing one in the afternoon. It was an SEM microscope, short for scanning electron microscope, that produces detailed images of extremely small particles by scanning them with a focused beam of electrons. There was one particular example image on display, where the microscope was able to show the bumps and ridges on a single human hair. (see picture) The technology is extraordinary, and I feel so lucky to have seen such beautiful images of spectacularly small particles. (see pictures)
Human hair magnified 3,000x with the SEM

Images produced by the SEM

 

Day 3- “This Ain’t No Lifetime Movie”

On Thursday morning, I was back in room 2A with Judge Davidian.  The first order of business was talking care of a return of weapons case.  Because lawyers had worked out the negotiations beforehand, this case was over in a matter of five minutes because all that was left was the paperwork.  One of the same lawyers from Tuesday was there, and I wondered how often the same lawyers appeared in one courtroom.  Turns out that many of them had become a familiar face, whether the Judge liked it or not.  After that, Judge Davidian called up a couple that was scheduled on the docket, and asked their lawyers how long they expected the trial to take.  He asked this from the rest of the lawyers present, and told many of the people waiting to be back at a certain time based on their answers.  It was clear he didn’t want those people to stay in that room if they didn’t have to.  That was rare.  Most Judges make them wait in the room and suffer through trial upon trial while waiting for their decision.

Halfway through our cases, we encountered two people without lawyers.  Because nobody will go to jail if they lose in this courtroom, no public defenders were awarded, so people who cannot afford a lawyer go pro se.  Going pro se means that they testify as a witness and represent themselves in a court of law.  Judge Davidian would ask questions to all of the pro se people while they were testifying because they did not have a lawyer to guide them and often left important information out of their testimony.  Their opponent is allowed to cross-examine the witness another time after the judge questions them, to make it fair to the opposing side.

One of our cases involved negotiating a visitation schedule for a child.  In this case, both of the parents were on opposing sides, and distrust clearly ran deep through the relationship.  Neither client trusted each other, and even though it was clear from testimony that they were both credible, that credibility was constantly attacked.  One woman began throwing allegations around, even starting one of her sentences with “…This ain’t no Lifetime movie, but…” and then proceeding to spin outlandish tales.  From there on, I learned that pro se cross-examination is mostly just yelling at the opposing party and trying to prove crazy claims.

In the afternoon, I was back in the Justice Center in room 303 with a new judge, Judge Eagles.  She was new to me, because this was the first time we had met, but it turned out that she was not at all new to the court system.  Her dad had served on the N.C. Court of Appeals, and she followed in his footsteps.  Room 303 on a Thursday afternoon was Criminal Domestic Violence.  I was sitting at the clerk’s desk, and I noticed many of the files had green sticky notes on them.  When I asked, the clerk told me that a green sticky note meant they had to be brought from the jailhouse to testify because they hadn’t been able to bail out.  Once those cases were done, we dealt with video first appearances, where Judge Eagles literally video called people in the prison to notify them of their bond soon after they had committed the crime.  I realized that showed the extent that our court system valued people’s right to a speedy trial, even when court is lots of waiting for other people.

Image of all the law books in chambers.  SO MANY RULES AND STATUTES!

Day 3

I came into today very excited to experience something different as we were about to explore the more creative part of SAS, Corporate Creative in building U. We met Aaron and Ashley who spent half the day showing us projects and how they create advertisements and booths for SAS. After explaining that to us and showing us programs and projects for a few hours we attended a creative meeting where employees express concerns about their projects and ask for help. It was neat seeing how they all helped each other make design decisions. Before lunch, we went on the “scenics tour” which is to the warehouse where all the printing and wood workshop and art is! Immediately after we went to lunch in C again. Here we met Ed Harriss who would be with us for the rest of the day in the video production building V. Ed gave us a tour of the building which consisted of many studios and equipment. Our last part of the day was spent in his office talking about what his job consists of and him showing us some of his most recent computer graphics work. George and I are excited to experience the marketing branch for our fourth day at SAS.

 

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