Today I got some one on one time working with Dr. Hsu in the lab. We spent a few hours making organoids in the tissue culture lab to be transferred to the mouse laboratory to be injected into the mice and developed into complete cancer cell lines to observe their growth. Tomorrow, we have to work on plating the organoids to make sure they’re ready to be injected into the mice. What happens after they are injected is that upon observing the natural growth, different drugs are tested, and our job is to observe whether the mice respond to the drug or not. And a response to the drug isn’t necessarily positive as the mice can respond poorly to the drug causing other medical symptoms, or the cancer cells could continue to duplicate. But, how did we make the organoids? What we did was take a piece of tumor delivered from the OR and we soaked it in a solution at room temperature over the weekend. This allowed parts of the tumor to dissociate into the solution. What we had to do then was remove the surrounding liquid without removing any of the floating cancer tissue. After this process was completed multiple times (a cycle of pipetting), the organoids were left in the tube and ready to be distributed to petri dishes to be observed under the microscope. Upon observing the organoids, you could see that they looked like a group of clumped cells. 
