We started off the day by facetiming Dr. Kwong about cancer, trauma, and the process of training to be a specialist. It was startling how different each cancer case is. One thing I learned is how cancer is staged – 1-4, depending on how far it’s spread. Next, we watched a video on suturing and practiced suturing on bananas using braided silk thread pre-attached to needles. We practiced two types of sutures: interrupted and running.
After lunch, we looked at the results from the DNA experiment.
| # | Contents | – : no amp | + amp | ++ amp + arabinose |
| 1 | control – pGLO | a lot | confluence | confluence and they glow! |
| 2 | control: UC19 | n/a | confluence | n/a |
| 3 | Ligation: GFP | n/a | none | none |
Table 1. The results of the DNA experiment
The reason we didn’t have anything from tube three is that something went wrong during the ligation reaction – the error was not in the transformation, because the controls still worked. Even though tube 3 didn’t work, tube 1 in the ++ plate was very successful and glowed strongly.