This is the first independent project in Cary Academy ADV Biotechnology. Consider watching this video and commenting to provide feedback. Please focus on the science, information-explanation, and/or the video production. At the end of the video, the students ask questions to the viewers. Citations can be found on a separate document underneath the video or within the video.
Citations: works-cited_osteoporosis
Extremely well done presentation of the work: detailed background is given, the function of the protein translated and the experimental design are explained very well. I was wondering what the mutations are and how the resulting protein is impaired. Is there a three-dimensional structure of the protein?
Thank you so much for critiquing our video! Unfortunately we could not locate and X-ray crystallography of our protein, but have managed to find an image of Frizzled 4, a protein that works with LRP5 during WNT.
Thank you,
Patrick
Excellent video. High points: Experimental design, explanation of primer design, overview of osteoporosis and genes involved, and LRP5 protein function.
Video Production: Next steps- It is now time to add your final gel images with a results section describing PCR (Master Mix), PCR cycle, and electrophoresis. You could add the challenges that you encountered. Great: The audio on this video is very good but the rate of speech is FAST. Very good pronunciation. A photo at the beginning of the two of you would have been a nice addition. A better explanation of inheritance would also be a nice touch instead of quickly moving away from the pedigree.
Science: 1.) How is it that you will confirm that you got the correct amplicon other than the size? 2.) Within LRP5, what was the mutation that was targeted and is it in the active domain of the protein? – frameshift mutation? deletion? SNP-Point mutation? 3.) Providing an example gel and the expected size would be a nice addition- with expected controls. 4.) Now you should be able to provide your results and explain the gel/amplicon. Even if the amplicon was not confirmed by another method (other than size)- it seems the primers were very specific.
Video production – You explained basic information that would be necessary to follow the rest of your presentation in a manner that a non-science person could understand. A few charts were a little blurry on full-screen. You used an “ABCDE” collage of pictures but did not fully describe what each one was until about 3 minutes into the video. Pay attention to your rate of speech – maybe slow down just a bit. Also helpful to display questions on screen so viewer can reflect on them, rather than just vocalizing them.
You did a great job choosing primers based on their qualities.
Thank you for the critique, we definitely need to work on slowing down our speed of speech. We’ll be sure to make any future videos more visually-interactive and include questions as well as describing each image as it/they appear.
Your purpose was well documented in terms of what you were trying to do. The work is very superior and exceeds that of most undergrad students. Please keep up the fine work. Number your slides for viewer reference for discussion. Slide showing pathology of disease was used twice- perhaps that is not necessary and discussion of the two slides integrated.
Thank you for the positive feedback! We will continue to keep up the hard work and will work to not re-use pictures on future videos.